Journal: The Journal of Clinical Investigation

Article Title: Biomarkers of inflammation and repair in kidney disease progression

doi: 10.1172/JCI139927

Figure Lengend Snippet: Wild-type mice were subjected to 27 minutes of unilateral IRI with contralateral kidney intact (atrophy model) or unilateral IRI with contralateral nephrectomy (repair model). The mice were sacrificed on days 1, 7, 14, and 30 after injury. Contralateral, atrophy, and repair kidneys were harvested at 30 days after injury, and kidney sections were stained with Picrosirius red to detect collagen deposition and immunostained with anti-PDGFRβ antibody to detect interstitial myofibroblasts. (A and B) Representative images of kidney sections. Scale bars: 500 μm. (C–E) Quantification of cortical and medullary thickness, Picrosirius red area, and PDGFRβ-positive area, respectively. n = 10 kidneys/model. P < 0.0001 among group means and ***P < 0.001, ****P < 0.0001 by 2-way ANOVA in the indicated subgroup analyses. (F and G) Quantitative RT-PCR analysis for Col1a1, Fn1, Pdgfrb, Ccl2, Chi3l1, and Umod was performed on whole-kidney RNA harvested on days 0, 1, 7, 14, and 30 after injury. n = 10 kidneys/time point/model. Two-way ANOVA summarized in Supplemental Table 5. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 at the indicated time point. NS, not significant.

Article Snippet: PDGFRβ-positive cells were detected by IHC using primary monoclonal antibodies against PDGFRβ (3169, Cell Signaling Technology) as described previously ( 14 ).

Techniques: Staining, Quantitative RT-PCR